To evaluate consistency in psilocybin potency and to determine levels of microbial, pesticidal, and heavy metal content in products encompassing biomass of uniformly cultivated P. cubensis.

In a legally sanctioned, unique laboratory in Jamaica, we cultivated P. cubensisaccording to published methods, then dried, pulverized, extracted, and tested fruiting bodies for tryptamine content using an Agilent HPLC 1290 Infinity assembly. Colony counting was employed for E. coli, yeast, mold, and coliform presence, while a Neogen’s Veratox^® ELISA assay assessed mycotoxin content. Agilent GCMS and LC assemblies evaluated for pesticidal content while heavy metals (As, Cd, Pb, Hg) were determined using instrumental neutron activation analysis (INAA), energy-dispersive X-ray fluorescence (ED-XRF, and direct mercury analysis (DMA) by thermal decomposition–amalgamation–atomic absorption spectrometry (TDA-AAS), respectively.

Mean psilocybin and psilocin content in dried cultivated P. cubensis was 1.14 ± 0.17% by weight; however, there was batch variability, potentiating significant differences in projected dosage, particularly for and above 3 g. The homogenized biomass was deemed safe, with acceptable levels of microbial, mycotoxin, pesticidal, and heavy metal contents, and no significant carcinogenic or other health hazards. Encapsulated biomass stably maintained tryptamine content for 11 months.

Standardized, safe biomass suitable for human consumption can be achieved using P. cubensis cultivated under stringent, aseptic conditions. Given the observed variability, it is highly recommended that each batch is tested for tryptamine content. Our results may be useful for policymakers, cultivators, clinicians, and consumers.